Vascular Response to Isoproterenol and β2 Adrenergic Receptor Polymorphisms
Status:
Completed
Trial end date:
1969-12-31
Target enrollment:
Participant gender:
Summary
Single nucleotide polymorphisms at codon 46 and 79 of the gene encoding for the ß2 adrenergic
receptor (ß2AR) modify its pharmacological properties and may alter the response to ß2AR
agonists. The goal of the present study was to evaluate the role played by the Arg16Gly and
Gln27Glu polymorphisms on isoproterenol induced relaxation of internal mammary arteries
segments ex-vivo.
Internal mammary leftover segments were collected from 96 patients undergoing coronary artery
bypass graft operation. Four rings that were prepared from each specimen were allowed to
reach equilibrium with physiological Krebs solution prior to precontraction with U46619.
Using the organ bath technique, cumulative dose response curve of isoproterenol was
constructed and mean EC50 calculated for each patient.