The Effects of Lymphocyte Therapy on Pregnancy Rate of Patient With Recurrent Implantation Failure
Status:
Not yet recruiting
Trial end date:
0000-00-00
Target enrollment:
Participant gender:
Summary
Infertility and miscarriage ordinary events in reproductive failure in humans, as are
affected one couple in every six couples of reproductive age and abortion is including in
approximately 15-20% of all pregnancies. Over the decades since the beginning of Assisted
Reproductive Technology (ART) and in vitro fertilization (IVF) pregnancy rate still remains
below 30% and Recurrent Implantation Failure in one of the most important limiting factor is
the assisted reproductive techniques. According to studies conducted in recent years one of
the most important mechanisms of implantation failure is maternal immune system because the
fetus as an allograft toxic (Semi allograft) to the mother. Studies have demonstrated that
ratio of Th1 to Th2 cells increase in maternal peripheral blood cells can be directly
associated with implantation failure. Lymphocytes during implantation, in association with
endometrium and blastocyst, participate in the production of cytokines, in particular Th1
pre-inflammatory cytokines. TNFα, IFNγ ،LIF ،IL12 ،IL15 Among these are pre-inflammatory
cytokines that appear to be necessary in the early stages of implantation. The initial
inflammatory response should therefore be selectively reduced to preserve pregnancy.
Inflammatory response impairment at the beginning of pregnancy causes fetal implantation
failure, while high inflammation leads to acute rejection (spontaneous abortion) or chronic
(pre-eclampsia) ). Preserving pregnancy requires a specific cytokine pattern, in particular
Th2 cytokines (IL3, IL4, IL5, IL10, IL13, GMCSF), which causes the anti-inflammatory state of
pregnancy required. Therefore, maternal immunity seems to be involved in creating and
sustaining pregnancy through the Th1 / Th2 Thalassic Balance. An imbalance between the two
systems can be an explanation for the implantation failure in some patients. The effect of
intra-uterine lymphocyte therapy on the fertility rate of women with RIF in the mouse model
has been shown to be controlled by the Th1 system. While the Th2 system has a protective
role, two thirds of patients have had an incomplete endometrial admission. Since the immune
cells of the uterus are balanced through a broad cytokine pattern, it is important to
evaluate the implantation success of RIF patients after intrauterine insemination (IUI) of
their own lymphocytes in the uterus in the pre-implantation phase. Laboratory tests on mice
show an increase in the number of embryonic implantations with human PMBCs. Considering the
importance of TH1 and TH2 cells in the success or failure of pregnancy and the relationship
between the function and balance of these cells with pregnancy problems, such as implantation
failure (RIF), and the role of factors and inflammatory cytokines in creating proper nesting
conditions, by introducing the lymphocytes of a participants infected with RIF into the
uterus in order to induce primary inflammation, the investigators will examine its effect on
fertility and the success of implantation in women with RIF.