The Effect of Dehydration on Intestinal Permeability
Status:
Completed
Trial end date:
2018-06-21
Target enrollment:
Participant gender:
Summary
In this study, the effect of dehydration by sauna exposure on the intestinal permeability in
20 healthy subjects is investigated. Participants attend three visits: 1) Sauna visit (to
achieve 3% dehydration), 2) Positive control visit (intake of indomethacin which is known to
increase intestinal permeability), 3) Negative control visit. At all visits, saliva samples,
blood samples, faecal samples, saliva samples are collected and the multi-sugar permeability
test is performed. In this test, participants drink a sugar solution and then urine collect
urine for 5 and 24 h. The ratio of the sugars detected in the urine by liquid
chromatography/mass spectometry is a reflection of the intestinal permeability. Saliva
samples are collected for assessment of cortisol, a stress marker. Blood and faecal samples
are collected for assessment of markers of intestinal barrier function and inflammation.