Overview

Retapamulin Microdialysis Feasibility Study

Status:
Completed
Trial end date:
2014-05-27
Target enrollment:
0
Participant gender:
All
Summary
This feasibility study will allow for the determination of the in vivo recovery and time of dialysis to optimize a future thorough microdialysis study. This is a single session, open label study to evaluate the feasibility of microdialysis for Retapamulin in healthy subjects. Three healthy subjects will be enrolled and complete the study procedures. Subjects will be admitted to the research unit on Day 1 and three microdialysis probes will be placed in the thigh of each subject prior to the start of the microdialysis procedure. After normal saline solution infusion for 30 minutes, a Retapamulin solution will be infused for 90 minutes. Saline perfusion will occur during the washout period. Microdialysis sampling will be done for 30 minutes (during the last 30 minutes of drug perfusion) and dialysate sample collection will continue every 30 minutes for 4 hours. The approximate duration of study including follow-up is 4 days.
Phase:
Phase 1
Accepts Healthy Volunteers?
Accepts Healthy Volunteers
Details
Lead Sponsor:
GlaxoSmithKline
Treatments:
Retapamulin
Criteria
Inclusion Criteria:

Healthy as determined by a responsible and experienced physician, based on a medical
evaluation including medical history, physical examination, laboratory tests. A subject
with a clinical abnormality or laboratory parameters outside the reference range for the
population being studied may be included only if the Investigator and the GlaxoSmithKline
(GSK) Medical Monitor agree that the finding is unlikely to introduce additional risk
factors and will not interfere with the study procedures. Subjects with clinically
significant lab values outside the normal range should always be excluded from enrollment.

- The subject is able to understand and comply with protocol requirements, instructions
and protocol-stated restrictions and is likely to complete the study as planned.
Subject is willing to provide informed consent.

- Does not use medications containing Retapamulin while participating in the study.

- Male or female between 18 and 55 years of age inclusive, at the time of signing the
informed consent.

- Body Mass Index within the range 18.5 to 32 kilogram/ meter^2 (inclusive).

- The following normal ranges for blood pressure and heart rate are given as a guide:
Systolic blood pressure 90 to 140 milimeter of Mercury (mmHg), Diastolic blood
pressure 60 to 90 mmHg, and Heart rate 45 to 100 beats per minute (bpm). The
investigator may interpret screening vital sign data based on the subject's age,
physical state and level of fitness. Subjects with vital sign readings marginally
outside the normal range may be included in the study if in the investigator's opinion
these values are not clinically significant and will not present a safety risk or
affect study assessments.

- A female subject is eligible to participate if she is of non-childbearing potential
(defined as pre-menopausal females with a documented tubal ligation or hysterectomy;
or postmenopausal defined as 12 months of spontaneous amenorrhea [in questionable
cases a blood sample with simultaneous follicle stimulating hormone > 40 milli
international unit/mililiter (mL) and estradiol < 40 picogram/ml [<147
picomoles/Liter] is confirmatory]. Females on hormone replacement therapy and whose
menopausal status is in doubt will be required to use one of the contraception
methods) or Child-bearing potential and agrees to use one of the contraception methods
for an appropriate period of time (as determined by the product label or investigator)
prior to the start of dosing to sufficiently minimize the risk of pregnancy at that
point. Female subjects must agree to use contraception until study completion (i.e.
follow-up phone call). Male subjects with female partners of child-bearing potential
must agree to use one of the contraception methods. This criterion must be followed
from the time of the first dose of study medication until study completion (i.e.
follow-up phone call).

- Alanine aminotransferase, alkaline phosphatase and bilirubin <= 1.5x Upper limit of
normal (ULN) (isolated bilirubin >1.5xULN is acceptable if bilirubin is fractionated
and direct bilirubin <35 percent).

- QT interval corrected for Heart rate (QTc), QTcB (Bazett's formula) or QTcF
(Fridericia's formula) < 450 millisecond (msec); or QTc < 480 msec in subjects with
Bundle Branch Block.

- Does not currently smoke cigarettes or tobacco and has not smoked within the past 12
months prior to the study.

- Does not consume alcohol 72 hours prior to the start of and during the study

Exclusion Criteria:

- Unwillingness or inability to follow the procedures outlined in the protocol

- Subject is mentally or legally incapacitated.

- History of current significant medical illness including cardiovascular thrombotic
events, myocardial infarction, stroke or other cardiac disease, hypertension, peptic
ulcer disease or gastrointestinal bleeding, skin disorders, hematological disease,
bronchospastic respiratory disease, asthma, diabetes mellitus, renal or hepatic
insufficiency, or any other illness that the investigator deems clinically significant
for exclusion of the subject from the study.

- History of sensitivity to any of the study medications, or components thereof or a
history of drug or other allergy that, in the opinion of the investigator or GSK
Medical Monitor, contraindicates their participation.

- History of skin conditions including atopic dermatitis, dry skin, or ichtyosis
vulgaris.

- Surgical history within 3 months prior to the start of the study.

- Clinically significant acute illness 7 days prior to study start.

- Excessive hair, tattooing, scar tissue, or other tissue damage at the application site
that could affect drug recovery or patient safety.

- Use of topical moisturizers (does not include facial moisturizers) 48 hours before the
start of and during the study.

- Strenuous exercise 48 hours before the start of and during the study.

- Unable to refrain from the use of non-prescription drugs, including non- steroidal
ant-inflammatory drugs (NSAIDS), vitamins, herbal and dietary supplements (including
St John's Wort) within 7 days (or 14 days if the drug is a potential enzyme inducer)
or 5 half-lives (whichever is longer) prior to the first dose of study medication,
unless in the opinion of the Investigator and GSK Medical Monitor the medication will
not interfere with the study procedures or compromise subject safety.

- History of regular alcohol consumption within 6 months of the study defined as an
average weekly intake of >14 drinks for males or >7 drinks for females. One drink is
equivalent to 12 gram of alcohol: 12 ounces (360mL) of beer; 5 ounces (150mL) of wine;
or 1.5 ounce (45mL) of 80 proof distilled spirits.

- A positive pre-study drug/alcohol screen.

- Pregnant females as determined by positive serum human chorionic gonadotropin (hCG) at
screening or urine hCG test prior to dosing.

- Lactating females.

- The subject has participated in a clinical trial and has received an investigational
product within the following time period prior to the first dosing day in the current
study: 30 days, 5 half-lives or twice the duration of the biological effect of the
investigational product (whichever is longer).

- Exposure to more than four new chemical entities within 12 months prior to the first
dosing day.

- Where participation in the study would result in donation of blood or blood products
in excess of 500 mL within a 56 day period.

- Employees of the investigator or study center, with direct involvement in the proposed
study or similar studies including family members of the employees or the investigator