The purpose of the study is to determine the effect of a two-step mouth rinsing procedure on
reducing the germs (mutans Streptococci) that cause tooth decay. We hope that the two-step
mouth rinse (0.3% NaOCl followed by 10% Povidone Iodine) decreases the tooth causing germs
better than the one-step mouth rinse (10% Povidone Iodine). The mouth rinse for both
treatment groups is done only once at the beginning of the study after the initial saliva
samples are taken, and before a detailed examination of the teeth and gums. It is
hypothesized that the treatment group receiving the NaOCl and Povidone iodine will have
significantly lower microbial counts than the control group (Povidone iodine) because the
NaOCl will disrupt the microbial biofilm and make the Povidone iodine more effective in
decreasing the microbes in the biofilm. Microbial counts are made from Salivary samples taken
from participants at baseline, one week, one month, two months and three months. The samples
are tested by two commercial methods for determining microbial counts. The CariScreen Test is
a rapid bioluminescence assay that quantitates the total number of all of the organisms
present. The CRT test requires a culture media that is specific for the two germs (S. mutans
and Lactobacillus) strongly associated with tooth decay. The results of the CRT Test are read
after three days of incubation.