Plasma Holotranscobalamin as Compared to Plasma Cobalamins for Assessment of Vitamin B12 Absorption
Status:
Completed
Trial end date:
2005-10-01
Target enrollment:
Participant gender:
Summary
In the present study the design of the vitamin B12 absorption test, CobaSorb, is further
optimised. We investigate which test - measurement of holotranscobalamin or cobalamins -
could be used for reflection of vitamin B12 absorption. Furthermore, we prolong the duration
of vitamin B12 administration in order to determine the final duration of the vitamin B12
absorption test CobaSorb.
Seventy-eight healthy individuals (age 21-81 years) are treated with three oral doses of 9
microgram cyanocobalamin per day for five successive days. Non-fasting blood samples are
collected on day 1-5 before administration of the first dose of vitamin B12 and on day 8.
Cobalamins and holotranscobalamin are measured on day 1-5 and 8. The performance of the
vitamin B12 absorption test will be evaluated in individuals with borderline or low levels of
holotranscobalamin or cobalamins (below the 75% percentiles) using a change larger than
2CV(day to day) of holotranscobalamin (22%) and cobalamins (12%) to indicate a change caused
by absorption of the administered vitamin B12.
Phase:
N/A
Details
Lead Sponsor:
University of Aarhus
Treatments:
Hydroxocobalamin Vitamin B 12 Vitamin B Complex Vitamins