The aim of this study is to determine vitreous levels of nonsteroidal antinflammatory drugs
(NSAIDs) and prostaglandinE2 (PGE2) in eyes scheduled for vitrectomy.
All patients received the study drugs for 3 days before surgery. 0.5 to 1.0 mL of undiluted
vitreous was removed using the vitreous cutter positioned in the midvitreous cavity. Samples
were immediately frozen and stored at -80°C until analysis.
Sample analyses were performed using an high-performance liquid chromatography system. The
lower limit of quantitation was 0.8 ng/mL.
PGE2 levels were analyzed using the Prostaglandin E2 Monoclonal EIA Kit (Cayman Chemical
Company) according to the manufacturer's instructions.