Molecular Mechanism of POP Kidney Yin Deficiency Syndrome From the Interventional Effects of CLCF1
Status:
Unknown status
Trial end date:
1969-12-31
Target enrollment:
Participant gender:
Summary
This study use RT-PCR and Western Blot technique to detect the expression of CLCF1 mRNA and
protein in POP Kidney Yin deficiency group and healthy group to verify the relevance between
CLCF1 and POP the kidney Yin deficiency syndrome.Through siRNA and overexpression,observe the
mRNA and protein 's expression of CBP、JAK1、STAT4 and the protein phosphorylation of
JAK1、STAT4 in JAK-STAT signal to reveal the CLCF1 regulate the CBP's mechanism.Use the Liuwei
Dihuang Pills to detect the effect of treatment ,compare use or not the Liuwei Dihuang Pills
to detect the mRNA and protein 's expression of CLCF1、CBP etc.Aim to clarify the POP kidney
Yin deficiency molecular mechanism.