Long-term Release of Fluoride Formed on Enamel by Fluoride Gel or Varnish to Biofilm Fluid
Status:
Completed
Trial end date:
2016-12-01
Target enrollment:
Participant gender:
Summary
The anticaries effect of professional fluoride application has been associated with the
formation of calcium fluoride-like products ("CaF2") on dental surface, which may function as
a slow release fluoride reservoir. Among professional fluoride products, fluoride gel and
varnish are the most important, and have different soluble fluoride concentrations, pHs and
vehicles. Although the concentration of fluoride formed on enamel after the use of both is
similar, the retention of these reaction products is unknown. Also, it is unknown the
capacity of these reservoirs to enhance dental biofilm fluid with fluoride. Moreover, the
effect of the varnish application time on these parameters is unknown. Therefore, the aim of
this study is to compare the capacity of fluoride gel and varnish to form fluoride reservoirs
on enamel and increase dental biofilm fluid fluoride concentration with time. Rapid
biofilm-forming individuals will be selected to participate and divided into 4 experimental
groups: 1. Negative control: no treatment; 2. Varnish 4 hours: fluoride varnish will be
applied and kept on teeth for 4 hours; 3. Varnish 24 h: fluoride varnish will be applied on
teeth and kept for 24 h; and 4. Gel: Fluoride gel will be applied on teeth. Microbiopsies of
enamel will be obtained by a microbiospy technique to assess fluoride concentration before, 7
and 28 days after the treatments. Dental biofilm will be collected before and 3, 7, 14 and 28
days after treatments. Fluoride concentration on enamel and in the biofilm fluid will be
determined by an ion-specific electrode. Data will be analyzed statistically comparing the
groups and collection times.