Molecular testing of the gut microbiome and enteric pathogens is rapidly moving beyond
targeted PCR testing to next generation sequencing techniques. In addition, the current state
of monitoring for soil-transmitted helminth infections is moving increasingly from
microscopic techniques to molecular techniques. The targeted PCR test for soil transmitted
helminth diagnosis has been validated on stool samples, but not rectal swabs. Bulk stool
samples are logistically challenging and time-intensive to collect, thus participation is
often far from optimal. Rectal swabs are more efficient and may result in higher
participation. In this study, children will be randomized to either albendazole,
azithromycin, or both drugs, after which both rectal swabs and bulk stool samples will be
collected. The investigators will compare the PCR test for soil transmitted helminth
infections using both the rectal swabs and the bulk stool samples. In addition, the
investigators will analyze the gut microbiome of the children using next generation
sequencing techniques to gain insight into effects of mass drug administration.
Phase:
Phase 4
Details
Lead Sponsor:
Francis I. Proctor Foundation University of California, San Francisco