Immediate Effect and Substantivity of the Essential Oils Without Alcohol on the Oral Biofilm in Situ

• Status: Unknown status
• Trial end date: 1969-12-31
• Target enrollment: 0
• Participant gender: All

Summary

This study aims to evaluate the in situ antibacterial activity of a mouthwash containing essential oils with and without alcohol on undisturbed de novo plaque-like biofilm (PL-biofilm) up to 7 h after its application.

An appliance was designed to hold six glass disks on the buccal sides of the lower teeth, allowing PL-biofilm growth. Twenty healthy volunteers will the appliance for 48 h and then performed a mouthwash with essential oils. Disks will be removed after 30 s and at 1, 3, 5, and 7 h later. After a washout period, the same procedure will be repeated with a sterile water mouthwash and a mouthwash with essential oils without alcohol. After PL-biofilm vital staining, samples will be analyzed using a confocal laser scanning microscope analysing their bacterial viability and thickness.

• Phase: Phase 4
• Accepts Healthy Volunteers?: Accepts Healthy Volunteers

Details

• Lead Sponsor: University of Santiago de Compostela
• Treatments: Listerine; Sodium Fluoride

Criteria

Inclusion Criteria:

Systemically healthy adults.

• Minimum of 24 permanent teeth.

• No gingivitis (Community Periodontal Index score = 0).

• No periodontitis (Community Periodontal Index score = 0).

• Absence of untreated caries.

Exclusion Criteria:

• Smoker or former smoker.

• Presence of dental prostheses.

• Presence of orthodontic devices.

• Antibiotic treatment or routine use of oral antiseptics in the previous 3 months.

• Presence of any systemic disease that could alter the production or composition of saliva.