Ethanolic Extract of Sapindus Mukorrossi as Final Endodontic Irrigant.
Status:
Completed
Trial end date:
2021-09-01
Target enrollment:
Participant gender:
Summary
The chief aim of root canal treatment is to reduce the microbial content and prevent further
recontamination in the canal. Properly cleaned, shaped and obturated teeth will be having
fewer chances of treatment failure. Every tooth has variable, complex canal anatomy
consisting of ramifications and lateral canal. The instrumentation alone cannot reach all the
areas for cleaning. For proper cleaning and removal of all microorganisms, the canal should
be irrigated with any biocompatible, lubricant and antimicrobial agent. An irrigant should
have a high compatibility factor, with canals, with obturating material and sealer. It should
remove all the superficial barriers from canal tubules and allow sealer penetration, which
aids in better adhesion and decreases the chances for recontamination.
Objectives: This study aims to compare the effect of experimental endodontic irrigant
Sapindus mukorossi with 17% EDTA, on the microleakage, sealer penetration and interaction
with sodium hypochlorite.
Ninety single-rooted teeth will be allocated into 2 experimental groups and a control group
of 30 teeth each. The endodontic procedure will be done with protaper files. The sample in
group 1 will be irrigated with 3.0% NaOCl after that by 17% EDTA and the sample in group 2
final irrigation will be given with Ethanolic extract of Sapindus mukorossi. After the
preparation and final irrigation of all teeth, sterile saline will be used to flush all
canals and dried using paper points. Obturation will be done with lateral condensation by
gutta-percha. Teeth will be incubated at 37ºc and 100% humidity for 7 days. Varnish will be
applied on the root surface except for apical 3mm. The teeth will be sectioned longitudinally
and examined at 2 and 5mm from the root apex. The maximum depth of sealer penetration will be
measured using a scanning electron microscope and microleakage will be tested through a dye
penetration method under a stereomicroscope.
Testing of interaction between irrigant and hypochlorite will be done by placing 1ml irrigant
with 1 ml sodium hypochlorite in a round bottom test tube and left for 1 week at 37ºc. An
independent observer will look for precipitation formation or color changes