Effect of Using Different Concentrations of NaOCI on IL-8 Level
Status:
Not yet recruiting
Trial end date:
2022-07-20
Target enrollment:
Participant gender:
Summary
The objective is to compare various concentrations of NAOCL during Root canal treatment and
their effect on IL-8 at the gingival crevicular fluid. Despite the current advancement of
beneficial endodontic diagnostic methods, such as pulp sensitivity tests and periapical
radiographs, clinical outcomes do not always correspond with the arrangement of the
histological condition of the pulp. At this point, the need for other ways to help diagnose
pathological pulpal disease has arisen. This situation has influenced researchers to use
molecular evaluation as an alternative route in endodontic diagnosis. Biomarkers, functional
at the cellular and molecular level, are crucial elements within the pathological process.
Detection of molecular markers is considered an ancillary method for diagnosing the
pathological condition of the pulp tissue.An increase in inflammatory cells in the carious or
traumatized dentinal pulp complex has been reported. In the presence of bacteria, immune
cells produce interleukin-6 (IL6), interleukin-8 (CXCL8), and tumor necrosis factor-alpha
(TNF) in the pulp. Interleukin-8 is frequently expressed in endothelial cells of inflamed
pulp and rarely in the normal pulp. Karapanou et al. (2008) revealed that interleukin-8 was
more greatly released in teeth with irreversible pulpitis than in the control group in a
research in which gingival crevicular fluid with acute pulpitis was analyzed. the pulp tissue
and gingival crevicular fluid of teeth with symptomatic irreversible pulpitis were
dramatically augmented by these indicators compared with healthy teeth. Furthermore, they
concluded that the levels of NKA, SP, IL8, and MMP8 in the gingival crevicular fluid
decreased one week after endodontic treatment of teeth with pulpitis. Subsequently, it has
been reported that patients with symptomatic irreversible pulpitis with high initial pain
scores have higher levels of SP, IL8, and MMP8 in the pulp tissue samples than those with low
pain scores.It is recommended to use NaOCl concentration between 0.5% and 5.25% as an
irrigation solution in root canal treatment (6). The effectiveness of NaOCl increases with
increasing concentration, but its toxicity is known to be proportional to concentration.
Therefore, there is no consensus on the optimal concentration of NaOCL. When articles were
reviewed, it was found that inflammation of the pulp and biomarker levels are correlated. For
this reason, we believe that whether or not NaOCl solution at various concentrations causes
inflammation can be analyzed by the level of IL-8. Upon review of the literature, it was
found that no studies investigated the influence of NaOCl solution on biomarkers in the
gingival crevicular fluid. The research described below contributes to the literature on the
optimal concentration of NaOCl to use.