Effect of Physiologic Hyperglucogonemia on Adipocyte Metabolism
Status:
Recruiting
Trial end date:
2026-08-25
Target enrollment:
Participant gender:
Summary
Purpose/Objectives: To investigate the effect of hyperglucagonemia on insulin action,
particularly on adipose tissue.
Research Design/Plan: Normal glucose tolerant subjects will be studied. Study subjects will
receive a continuous glucagon infusion for 12 hours. Following glucagon infusion, subjects
will receive prime-continuous tracer infusions for additional 4 hours to measure adipocyte
metabolism. Within 6-8 weeks, subjects will return for a repeat study with normal saline as a
control group.
Methods: All subjects will have an oral glucose tolerance test prior to participation to
confirm they are normal glucose tolerant. Subjects will be admitted to the CRC at 4 PM and
will receive a continuous glucagon for 12 hours. At 6 AM on the following morning, subjects
will receive prime-continuous tracer infusions of the following for 4 hours (14C-glycerol,
3-3H glucose, and D2O). At 10 AM continuous indirect calorimetry will be performed to
determine rates of energy expenditure and glucose/lipid oxidation for 40 minutes. At 6 AM a
surgical biopsy of abdominal adipose tissue will be performed for measurement of adipocyte
metabolism. At 8 AM, the study team will infuse insulin/glucose to test for insulin
sensitivity.
Clinical Relevance: The results of this study will help the study team to further understand
the pathophysiology of metabolic disturbances that is induced by hyperglucagonemia in type 2
diabetes patients.
Phase:
Early Phase 1
Details
Lead Sponsor:
The University of Texas Health Science Center at San Antonio