Effect of Folic Acid on the Presentation of Nuclear Abnormalities in People With a History Drug Abuse
Status:
Not yet recruiting
Trial end date:
2023-08-01
Target enrollment:
Participant gender:
Summary
Background: Multiple studies have pointed to the harmful potential of licit and illicit
drugs.
as agents associated with neoplastic processes and other non-communicable diseases, for which
reason It has become a problem of global size. Objective: The central objective is to
determine the cytogenotoxic damage in the oral mucosa of people with chronic drug use, as
well as establishing the therapeutic effect of acid administration folic on said damage.
Methodology: Quasi-experimental study, pretest-posttest design with no control group.
equivalent, in subjects with substance abuse and healthy subjects. The intervention will
consist of administration of 15 mg of folic acid divided into 3 doses per day. Mucosal
samples will be taken orally in duplicate to each participant to determine the frequency of
micronuclei (MN), bursts cells (NBUD), binucleated cells (BN), condensed chromatin (CC),
karyorrhexis (CR), pyknosis (PIC) and caryolysis (CL) at different time events:
pre-treatment, 15 days and 30 days. So as a survey to determine consumption patterns of
psychoactives, sociodemographic data, dietary and exposure to known cytogenotoxic agents.
Resources and infrastructure: The study will be carried out in the pharmacology laboratory of
the Center University of Tonalá, who will make their equipment and reagents available. Group
experience: The research group has over a decade of experience in the development of projects
related to mutagenesis and cyto-genotoxic agents.
Development time: The project will be developed from February 2023 to August of 2023