Effect of Calcium Glycerophosphate (CaGP) - Fluoride Dentifrice on Dental Biofilm pH
Status:
Completed
Trial end date:
2008-07-01
Target enrollment:
Participant gender:
Summary
The aim of this study was to investigate the buffering effect of a calcium
glycerophosphate-fluoride (CaGP-F) dentifrice on in vivo dental biofilm after a cariogenic
challenge and evaluate its probable 12-hour protective effect. Twelve young adults took part
in this randomized, double blind, 14-day 4-phase crossover study. Between each phase, the
volunteers had a 1-week wash-out period. Coded dentifrices were randomly assigned to the
volunteers: A) no F and no CaGP; B) CaGP-only (0.13%); C) F-only (1500 ppm (ppm= parts per
million of fluoride which is equivalent to mg/kg)); D) CaGP-F (0.13%, 1500 ppm,
respectively). The pH measurements were taken from a single-site using a microelectrode, with
salt bridge established by a 1M KCl (one molar potassium chloride) solution with a reference
electrode. pH measurements were taken at 0 (baseline), 1, 7, 14 and 21 min (minutes) after a
cariogenic challenge (10% w/v sucrose solution, %w/v = percent weight per volume). Four sets
of measurements were carried out: (D0BS) before test dentifrice usage; (D01min) 1-min after
test dentifrice usage; and (D712h) 7 days and (D1412h) 14 days using the test dentifrice, 12
hours (h)after brushing. Stephen curves and mean AUC (area under the curve) were obtained.
Phase:
Phase 1
Details
Lead Sponsor:
Federal University of Paraíba
Collaborators:
Conselho Nacional de Desenvolvimento Científico e Tecnológico Coordination for the Improvement of Higher Education Personnel