Detection of miRNAs in HPA Axis Function in Healthy Subjects
Status:
Terminated
Trial end date:
2019-07-24
Target enrollment:
Participant gender:
Summary
The proposed study will test dexamethasone effect on miRNA profile in healthy humans. As our
data indicate that miRNA levels change over time, the investigators will study the time
course of miRNA response to 1 mg IV dexamethasone within 60 minutes after injection.
Dexamethasone is used routinely in human subjects for the treatment of inflammatory
conditions and as a diagnostic tool in the evaluation of excess cortisol secretion (Cushing's
syndrome). For the latter, 1 mg dexamethasone is given orally at midnight. Oral formula
absorption in the gut can range from 20 to 60 minutes due to the presence of food and other
factors. IV administration will bypass this variability and ensure an accurate and
reproducible time-course study. The investigators will compare miRNA expression before and
after dexamethasone treatment. The investigators will follow miRNA expression dynamics over a
1-hour time course to identify peak expression levels and to correlate miRNA expression with
circulating dexamethasone levels. Dexamethasone and miRNA levels will be checked at baseline
just before injection and then at 15, 30, 45, and 60 minutes after injection. Dexamethasone
level is taken to verify treatment and to correlate it with miRNA expression.