Detection of Acetylsalicylic Acid and Omega-3 Fatty Acids in Schirmers' Test Strips Using Mass Spectrometry
Status:
Completed
Trial end date:
2021-11-30
Target enrollment:
Participant gender:
Summary
Evaluation of tear fluid production using Schirmers' tests is a well-known and frequently
used examination method in ophthalmology. Schirmers' test objectively indicates the tear
fluid production over five minutes by insertion of a filter paper strip in the lower
conjunctival fornix. Commonly, this method is used to quantify tear fluid production, but not
tear fluid composition. The development of novel, very precise analytical methods opens up
new possibilities in the use and application of Schirmers' test strips. A recently introduced
analytical method is mass spectrometry. This method allows the detection and quantification
of proteins, lipids, and metabolites in very low amounts of samples. Whether systemically
ingested agents such as omega-3 fatty acids or acetylsalicylic acid can be detected in tear
fluid using this method remains unclear. The aim of this study is to investigate the
detectability of 2 different agents (acetylsalicylic acid and omega-3 fatty acids) in
Schirmers' test strips from healthy subjects after intake for 1 week by use of untargeted
mass spectrometry. Participating healthy subjects will receive either acetylsalicylic acid or
omega-3 fatty acids.