Elegible patients were included in the study and underwent treatment with a solution of
citicoline 1% eye-drops, 0.2% high molecular weight hyaluronic acid and 0.01% benzalkonium
chloride prior to surgery. The vitreous samples were taken at the beginning of the surgery
and analyzed for qualitative/quantitative determination of vitreous concentration of
citicoline and its metabolites by means of high performance liquid chromatography.