Overview

A Study of CAR-GPRC5D in Patients With Relapsed/Refractory Multiple Myeloma or Plasma Cell Leukemia

Status:
Not yet recruiting
Trial end date:
2023-03-01
Target enrollment:
0
Participant gender:
All
Summary
This study is a single-center, open, dose-escalation study to observe the safety and efficacy of different doses of CAR-GPRC5D in patients with R/R MM or plasma cell leukemia.
Phase:
Phase 1
Accepts Healthy Volunteers?
No
Details
Lead Sponsor:
Huazhong University of Science and Technology
Collaborator:
Nanjing IASO Biotechnology Co., Ltd
Criteria
Inclusion Criteria:

- Subjects must satisfy all the following criteria to be enrolled in the study:

1. age 18 to 75 years old, male or female.

2. Subjects have had at least 3 prior lines of therapy including chemotherapy based
on proteasome inhibitors (PIs) and immunomodulatory agents (IMiDs).

According to the International Myeloma Working Group (IMWG) consensus (2016)
standard on multiple myeloma, the disease has recurred, progressed or is
refractory, or according to the IMWG consensus (2013) standard on plasma cell
leukemia (Appendix 4), the disease appears relapse, progress or refractory;

3. Evidence of cell membrane GPRC5D expression, as determined by a validated
immunohistochemistry (IHC) or flow cytometry of tumor tissue (e.g., bone marrow
biopsies, or plasmacytoma).

4. The subjects should have measurable disease based on at least one of the
following parameters:

The proportion of primitive immature or monoclonal plasma cells detected by bone
marrow cytology, bone marrow biopsy, or flow cytometry is ≥ 10%.

Serum M-protein ≥ 0.5 g/dL. Urine M-protein ≥ 200 mg/24 hrs. For those whose
Serum or Urine M-protein does not meet the measurable criteria but the light
chain type, serum free light chain (sFLC) : involved sFLC level ≥ 10mg/dL (100
mg/L) provided serum FLC ratio is abnormal.

In subjects with extramedullary myeloma, if there are no other evaluable lesions,
require extramedullary lesions with a maximum diameter of ≥2cm

5. ECOG performance score 0-2.

6. Estimated life expectancy ≥ 12 weeks.

7. Subjects should have adequate organ function:

- Hematology: Absolute neutrophil count (ANC) ≥1×10^9 /L (prior use of growth
factor support is permitted, but subjects must not have received supportive
treatment within 7 days prior to laboratory examination); absolute
lymphocyte count (ALC) ≥0.3×10^9 /L; platelets ≥40×10^9 /L (subjects must
not have received blood transfusion support within 7 days prior to
laboratory examination); hemoglobin ≥60 g/L (subjects must not have received
transfusion of red blood cells [RBC] within 7 days prior to laboratory
examination; the use of recombinant human erythropoietin is permitted).

- Liver function: Alanine aminotransferase (ALT) and aspartate
aminotransferase (AST) ≤ 2.5×upper limit of normal (ULN); total serum
bilirubin ≤ 1.5×ULN.

- Renal function: Creatinine clearance rate (CrCl) calculated according to
Cockcroft-Gault formula ≥ 40 ml/min.

- Coagulation function: Fibrinogen ≥ 1.0 g/L; activated partial thromboplastin
time (APTT) ≤ 1.5×ULN, prothrombin time (PT) ≤1.5×ULN.

- SpO2 > 91%.

- Left ventricular ejection fraction (LVEF) ≥ 50%.

8. The subject and his/her spouse agree to use an effective contraceptive tool or
medication (excluding safety period contraception) for one year from the date of
the subject's informed consent to the date of CAR T cell infusion.

9. Subject must sign the informed consent form approved by ethics board in person
before starting any screening procedure.

Exclusion Criteria:

- The presence of any of the following will exclude a subject from enrollment:

1. Subjects who are known to have GVHD or need long-term immunosuppressive therapy.

2. Subjects have received any anti-cancer treatment as follows:

monoclonal antibody for treating multiple myeloma within 21 days before
leukapheresis, or cytotoxic therapy or proteasome inhibitors within 14 days
before leukapheresis, or immunomodulatory agents within 7 days before
leukapheresis. or anti-tumor treatments other than those listed above within 30
days before leukapheresis.

3. Subjects who were receiving a used therapeutic dose of corticosteroid treatment
(defined as prednisone or equivalent > 20mg) within 7 days prior to screening,
except for physiological alternatives, inhalation, or topical use.

4. Subjects with hypertension that cannot be controlled by medication.

5. Subjects with serious heart disease: including but not limited to unstable
angina, myocardial infarction (within 6 months prior to screening), congestive
heart failure (NYHA classification ≥III), and severe arrhythmias.

6. Subjects with systemic diseases that the investigator determined to be unstable
include, but are not limited to, severe liver and kidney or metabolic diseases
requiring medical treatment.

7. Subjects with second malignancies in addition to MM within the past 5 years
before the screening, exceptions to this criterion: successfully treated cervical
carcinoma in situ and non-metastatic basal or squamous cell skin carcinoma, local
prostate cancer after radical surgery, and ductal carcinoma in situ of the breast
after radical surgery.

8. Subjects with a history of organ transplantation.

9. Subjects have received major surgery within 2 weeks prior to leukapheresis or
plan to receive surgery during the study or within 2 weeks after the study
treatment (excluding local anesthesia).

10. Subjects participated in another interventional clinical study 1 months before
signing the informed consent (ICF);

11. Subjects with any uncontrolled active infection needed to receive systemic
therapy within 7 days before leukapheresis collection (excluding # CTCAE grade 2
urogenital infection and upper respiratory infection).

12. Positive for any of the following tests:

- Hepatitis B virus (HBV) surface antigen (HBsAg) or hepatitis B core
antibody-positive and detectable HBV DNA in peripheral blood

- Hepatitis C virus (HCV) antibody and hepatitis C virus RNA in peripheral
blood

- Human immunodeficiency virus (HIV) antibody

- Cytomegalovirus (CMV) DNA

- Treponema Pallidum antibody

13. Pregnant or lactating women.

14. Subjects with mental illness or consciousness disorder or disease of the central
nervous system

15. Other conditions that researchers consider inappropriate for inclusion.